HALCYONE Femto

Fluorescence Upconversion Spectrometer

Fluorescence Spectral Range: 270-1600 nm
Computer-Controlled

HALCYONE Femto is a fully automated Fluorescence Upconversion Spectrometer. It features our patented automated beam alignment system and can measure fluorescence decays with femtosecond time resolution over an 8 ns time window.

Features & Specs

Broad fluorescence spectral range

An exact fluorescence detection range depends on the laser wavelengths used for excitation and gating as the scattered laser light can interfere with the fluorescence signal. While the excitation wavelength is typically tuned to match the absorption of a specific chromophore, the gate wavelength normally corresponds to the fundamental output of the laser.

The residual excitation light after the sample can usually be blocked with an optical filter. However, the detection of the upconverted fluorescence signal is often obscured by the scattered gate and its harmonics leaking into the detector.

Therefore, for Ti:Sapphire lasers with the center wavelength of 800 nm detecting the fluorescence around 267 nm, 400 nm, and 800 nm is very difficult. It is similar for Yb lasers with the 1030 nm center wavelength – the fluorescence around 343 nm, 515 nm, and 1030 nm is hard to measure.

One way to mediate such spectral “blind spots” is to use an OPA for gate pulse generation.

Fluorescence can be measured across the following spectral ranges:

Spectral Range

With Ti:Sapphire laser (center λ ~800 nm)

With Yb laser (center λ ~1030 nm)

With optional gate OPA

UV

270 – 350 nm

260 – 340 nm

VIS

450 – 770 nm

370 – 950 nm

340 – 1000 nm

NIR

> 850 nm

> 1100 nm

Nanosecond time window

An up to 8 ns time window is covered by using an optical delay line. Our delay line features a low GVD dielectric retroreflector for high optical throughput and a shorter IRF; a direct drive linear motor for fast scanning; as well as computer-controlled steering mirrors for true hands-off operation.

Optical delay line specifications:

  • Time window: 8 ns

  • Resolution: 14 fs

  • Minimum step size: 2.8 fs

  • Max. translation speed: >10 ns/s

  • Self-alignment time: 3-5 min

  • Beam pointing drift: <10 µm across the time window

Detectors

HALCYONE is available with two detector options: single wavelength PMT or multi-wavelength CCD.

At any time HALYCONE can be upgraded to include the other detector option to extend the capabilities of the instrument.

Single photon counting PMT

with a monochromator. This detector enables HALCYONE to measure a single wavelength fluorescence signal at a particular time delay. The fluorescence signal can be measured as a function of time or wavelength by scanning an optical delay line or a computer-controlled monochromator, respectively. To maximize the sensitivity at each fluorescence wavelength, the phase matching angle of a non-linear crystal angle is automatically adjusted by a computer-controlled rotation stage.This PMT is capable of detecting single photons and offers the highest sensitivity. However, it can register only one photon per laser pulse and therefore is most beneficial at higher rep rates (tens of kHz and above). We recommend this detector for experiments where only select kinetics or spectra need to be acquired (no full time-wavelength-intensity surface is needed), and a high rep rate laser is available.

Thermoelectrically cooled CCD camera

(1024×255 pixels) with a high throughput imaging spectrograph. While not quite as sensitive as a single photon counting PMT, this detector can capture a broad spectrum at once and also register multiple photons per pixel, per laser pulse, thus providing a large dynamic range even at lower rep rates (~1 kHz). We recommend this detector for experiments where it is necessary to monitor the fluorescence spectral evolution as well as resolve decays at individual wavelengths. For example, energy transfer processes, solvation dynamics, vibrational relaxation, etc.

Computer controlled non-linear crystal

To maximize the upconverted signal intensity the non-linear crystal needs to be rotated to a certain angle, depending on the fluorescence wavelength. HALCYONE automatically adjusts this angle during the experiment, so no input from the user is needed.

Imaging monochromator

Halcyone utilizes an imaging monochromator, which was designed and optimized for time-resolved spectrometry.

This monochromator features high spectral resolution, as well as an astigmatism-corrected design, optimal for multipixel array detectors. With the dual exit ports, this instrument can be used as a spectrograph as well as a monochromator.

The monochromator’s design (300 mm, F#6) matches the optics in the rest of the setup and maximizes the overall spectrometer’s performance.

The monochromator features a 4-grating turret with kinematic mounts for optimal wavelength coverage, optical throughput, and spectral resolution.

The UV-enhanced Al coatings on the monochromator mirrors ensure high efficiency of fluorescence and upconverted signal collection.

Software

Halcyone features versatile and user-friendly LabVIEW based software for instrument control and data acquisition. The software allows for full experiment automation, so no input from the user is required for the whole experiment duration.

The software is also very user-friendly and versatile:

  • Supports a PMT detector with a computer-controlled monochromator.

  • Supports a CCD detector with a high throughput spectrograph.

  • Takes into account the pump and fluorescence wavelengths and automatically adjusts the computer-controlled non-linear crystal’s angle.

  • Automated alignment of the optical delay line.

  • Computer-controlled switching between UV, VIS, and NIR fluorescence spectral ranges.

  • Supports computer-controlled translating sample holder.

  • Supports pump beam shutter.

  • Supports motorized filter wheel for automated pump intensity control.

  • Saves every individual kinetic scan, so if the experiment is aborted (due to laser fluctuations, power outages, etc.) all previous scans are not lost.

  • Threshold adjusted automatic probe intensity spike rejection – an advanced setting that collects data points again if the probe is not stable.

  • API (Application Programming Interface) for HALCYONE is provided for further experiment customization and integration with external applications.

Applications

Time-resolved fluorescence spectrometers are designed to measure the lifetime of the emissive excited state of a molecule. As a result of the photon absorption, the molecule passes from the ground state to an excited state. The reverse transition will occur at a rate specific to this chromophore. The lifetime of fluorescence is unique for each molecule and depends on the molecule’s environment. This property makes time-resolved spectrometry a powerful tool for studying processes that may involve:

Radiative relaxation (fluorescence)

Vibrational relaxation

Internal conversion

Energy transfer

Intersystem crossing (i.e. singlet to triplet transition)

Solvation

Conformational changes

Electron transfer to another molecule or the solvent

In a broader sense, HALCYONE can be used in:

Photophysics

Cell biology

Photochemistry

Materials science

Photobiology

Nanoscience

Brochures & Examples

HALCYONE Femto Brochure

HALCYONE (CCD). Fluorescence of Coumarin 500.

HALCYONE (PMT). Fluorescence Of Coumarin 480.

HALCYONE (CCD). Fluorescence Of DHAQ.

HALCYONE (CCD). Fluorescence Of Flavin Mononucleotide.

HALCYONE (PMT). Fluorescence Of DCM (Anisotropy).

HALCYONE (CCD). Fluorescence of PTP in UV (275-360 nm).

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